significance of microrna targeted estrogen receptor in male fertility
Authors
abstract
objective(s): estrogen receptor-alpha (erα) mediates estrogen action in regulation of different levels of the hypothalamic-pituitary-testis axis. it has a key role in spermatogenesis. estrogen receptor alpha knock-out (er koα) male mice were infertile and severe impairment in spermatogenesis and seminiferous tubules was observed. recently, it has been reported that microrna (mirna) mir-100 and let-7b were predicted to target erα gene. mirna are small, endogenous, single stranded rna molecules that regulate gene expression and have been implicated in various disease states. it has been proved that some mirnas expression is tissue- and disease-specific, giving potential for identifying mirnas as a diagnostic tool. materials and methods: in this study, the change in the expression levels of mir-100, let-7b and erα expression levels were evaluated in oligospermic infertile patients (n=43) compared to control fertile subjects (n=43). after washing and separating sperms, total rna was isolated and then cdna was synthesized. the expression levels of mir-100 and let-7b and erα were evaluated by real time pcr. results: mir-100, let-7b levels were significantly higher than those in control group (p=0.008 and p=0.009, respectively). we have found that, erα level was significantly decreased in comparison with normal group (p< 0.0001). conclusion: changes in mir-100, let-7b and erα expression levels in oligospermic patients may be associated with the susceptibility and progression of infertility. the results of this study indicate that mirna can have a key role in spermatogenesis and might have a diagnostic and prognostic value in men infertility.
similar resources
Significance of microRNA targeted estrogen receptor in male fertility
Objective(s): Estrogen receptor-alpha (ERα) mediates estrogen action in regulation of different levels of the hypothalamic-pituitary-testis axis. It has a key role in spermatogenesis. Estrogen receptor alpha knock-out (ER koα) male mice were infertile and severe impairment in spermatogenesis and seminiferous tubules was observed. Recently, it has been reported that microRNA (miRNA) mir-100 and ...
full textSignificance of microRNA targeted estrogen receptor in male fertility
OBJECTIVE(S) Estrogen receptor-alpha (ERα) mediates estrogen action in regulation of different levels of the hypothalamic-pituitary-testis axis. It has a key role in spermatogenesis. Estrogen receptor alpha knock-out (ER koα) male mice were infertile and severe impairment in spermatogenesis and seminiferous tubules was observed. Recently, it has been reported that microRNA (miRNA) mir-100 and l...
full textEstrogen receptor gene 1 expression in caprine and its effect on fertility
The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of E...
full textEffect of estrogen receptor-subtype-specific ligands on fertility in adult male rats.
Maintenance of normal male fertility relies on the process of spermatogenesis which is under complex endocrine control by mechanisms involving gonadotropin and steroid hormones. Although testosterone is the primary sex steroid in males, estrogen is locally produced in the testis and plays a very crucial role in male fertility. This is evident from presence of both the estrogen receptors alpha (...
full textTargeted disruption of the estrogen receptor gene in male mice causes alteration of spermatogenesis and infertility.
The reproductive system of male mice homozygous for a mutation in the estrogen receptor (ER) gene (ER knock-out; ERKO) appears normal at the anatomical level. However, these males are infertile, indicating an essential role for ER-mediated processes in the regulation of male reproduction. Adult ERKO male mice have significantly fewer epididymal sperm than heterozygous or wild-type males. Althou...
full textEffects of Bisphenol A and Diethylstilbestrol on Estrogen Receptor Expression and Male Fertility
(traduction) On définit l’infécondité comme l’incapacité d’un couple à concevoir un enfant après un an de rapports sexuels sans protection. Les pathologies masculines de reproduction sont la principale cause d’au moins 20% des cas d’infécondi- té, si bien que l’infécondité masculine est un problème important pour la san- té globale de la population. Les agents perturbateurs endocriniens (APE)...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of basic medical sciencesجلد ۱۷، شماره ۲، صفحات ۸۱-۸۶
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023